Efficacy of Roncolab mobile application for diagnosing the primary sign of sleep-disordered breathing (snoring) in children / Eficacia de la aplicación móvil Roncolab para el diagnóstico de signo primario de trastorno respiratorio del sueño (ronquido) en niños

ABSTRACT Sleep-disordered breathing (SDB) is a group of disorders associated with breathing anomalies during sleep. Easily detectable by sound, snoring is one of the most common manifestations and the main sign of SDB. Snoring is characteristic of breathing sound during sleep, without apnea, hypoventilation, or interrupted sleep. It may reduce the percentage of sleep and increase microarousals due to breathing effort or gas exchange. A range of questionnaires have been validated and adapted to the pediatric population to screen for patients who require laboratory testing. The Pediatric Sleep Questionnaire (PSQ) screens for SDB and identifies primary signs such as snoring. RoncoLab is a mobile application that records and measures snoring intensity and frequency Aim To compare the RoncoLab app and the PSQ regarding how efficiently they diagnose snoring Materials and Method This was an observational, analytical study of 31 children aged 7 to 11 years who visited the pediatric dental clinic at Benemérita Universidad Autónoma de Puebla, Mexico (BUAP). The PSQ was applied to diagnose SDB. Guardians were then instructed on how to download and use the mobile application to record data while the child was sleeping at home. Agreement between RoncoLab and the PSQ was analyzed statistically by Cohen's Kappa index at 95% confidence level Results The Kappa index for identification of primary snoring was 0.743 (p<0.05). App sensitivity was 0.92, and specificity 0.82 Conclusion There is good agreement between PSQ and RoncoLab for diagnosing primary snoring, with acceptable sensitivity and specificity.

RESUMEN Los trastornos respiratorios del sueño (TRS) son un grupo de padecimientos asociados con anormalidades respiratorias del sueño. Una de las manifestaciones más comunes es el ronquido; signo fácil de detectar por el ruido que emite y se considera como el signo principal. Este trastorno es característico del ruido respiratorio durante el sueño, sin apneas, hipoventilación, ni interrupciones del sueño. Este puede ocasionar disminución del porcentaje del sueño y aumentar los microdespertares, esto debido al esfuerzo respiratorio o al intercambio de gases. En la actualidad existen cuestionarios validados y adaptados para la población pediátrica útiles como herramienta clínica para el tamizaje y selección de pacientes que requieran pruebas de laboratorio. El Pediatric Sleep Questionnaire (PSQ) permite el cribado de TRS e identificación de signos primarios como el ronquido. RoncoLab es una aplicación móvil que registra y mide la intensidad y frecuencia del ronquido. Objetivo Comparar la eficacia del diagnóstico del ronquido por medio del RoncoLab contrastado con el PSQ Materiales y Método Estudio observacional, analítico, en el cual se incluyeron 31 niños de 7 a 11 años, que acudieron a la clínica de odontopediatría de la Benemérita Universidad Autónoma de Puebla, México (BUAP) Se aplicó el PSQ a los 31 niños para el diagnóstico de TRS, después se le instruyó al tutor como descargar y utilizar la aplicación móvil para registrar los datos obtenidos en las horas de sueño en casa. El análisis estadístico de concordancia entre los instrumentos diagnósticos se realizó con el Índice Kappa de Cohen a un nivel de confianza del 95% Resultados El Índice de Kappa para la identificación de los ronquidos primarios fue de 0.743 (p<0.05). La sensibilidad de la aplicación fue de 0.92, mientras la especificidad fue de 0.82 Conclusión Existe buena concordancia entre el PSQ y el RoncoLab en el diagnóstico de ronquido primario, con sensibilidad y especificidad aceptable.

Quantification of the environmental impact arising from the utilization of whole and defatted Okara in fermentative and dehydration processes.

Okara is the insoluble pulp that remains after the grinding and filtration of soybeans during the production of soymilk and tofu. As it retains a noteworthy quantity of nutrients, there has been an increasing emphasis in the utilization of this residue for the development of sustainable processes. This study focused on assessing the environmental impact of employing okara as a medium for fermenting and dehydrating probiotic bacteria at laboratory scale. The evaluation was carried out using the Life Cycle Assessment (LCA) methodology, considering the entire process lifecycle. Whole okara and defatted okara were used as culture media for Lactiplantibacillus plantarum CIDCA 83114, followed by dehydration (either freeze-drying or spray-drying) and subsequent storage. For the purpose of comparison, both scenarios (whole and defatted okara) were evaluated using 1 kg of dehydrated final product for storage, as functional unit. Based on experimental results, the conservation of the received okara and the dehydration-storage (e.g., freezing and freeze-drying) phases were identified as the most significant environmental hotspots responsible for the most substantial impacts of the processes. The use of LCA facilitated the measurement of the environmental effects linked to the reutilization of okara as an agro-industrial residue, thus providing quantitative support when engineering its sustainable valorization.

Efficacy of Roncolab mobile application for diagnosing the primary sign of sleep-disordered breathing (snoring) in children.

Sleep-disordered breathing (SDB) is a group of disorders associated with breathing anomalies during sleep. Easily detectable by sound, snoring is one of the most common manifestations and the main sign of SDB. Snoring is characteristic of breathing sound during sleep, without apnea, hypoventilation, or interrupted sleep. It may reduce the percentage of sleep and increase microarousals due to breathing effort or gas exchange. A range of questionnaires have been validated and adapted to the pediatric population to screen for patients who require laboratory testing. The Pediatric Sleep Questionnaire (PSQ) screens for SDB and identifies primary signs such as snoring. RoncoLab is a mobile application that records and measures snoring intensity and frequency. Aim: To compare the RoncoLab app and the PSQ regarding how efficiently they diagnose snoring. Materials and Method: This was an observational, analytical study of 31 children aged 7 to 11 years who visited the pediatric dental clinic at Benemérita Universidad Autónoma de Puebla, Mexico (BUAP). The PSQ was applied to diagnose SDB. Guardians were then instructed on how to download and use the mobile application to record data while the child was sleeping at home. Agreement between RoncoLab and the PSQ was analyzed statistically by Cohen's Kappa index at 95% confidence level. Results: The Kappa index for identification of primary snoring was 0.743 (p<0.05). App sensitivity was 0.92, and specificity 0.82. Conclusion: There is good agreement between PSQ and RoncoLab for diagnosing primary snoring, with acceptable sensitivity and specificity.

Los trastornos respiratorios del sueño (TRS) son un grupo de padecimientos asociados con anormalidades respiratorias del sueño. Una de las manifestaciones más comunes es el ronquido; signo fácil de detectar por el ruido que emite y se considera como el signo principal. Este trastorno es característico del ruido respiratorio durante el sueño, sin apneas, hipoventilación, ni interrupciones del sueño. Este puede ocasionar disminución del porcentaje del sueño y aumentar los microdespertares, esto debido al esfuerzo respiratorio o al intercambio de gases. En la actualidad existen cuestionarios validados y adaptados para la población pediátrica útiles como herramienta clínica para el tamizaje y selección de pacientes que requieran pruebas de laboratorio. El Pediatric Sleep Questionnaire (PSQ) permite el cribado de TRS e identificación de signos primarios como el ronquido. RoncoLab es una aplicación móvil que registra y mide la intensidad y frecuencia del ronquido. Objetivo: Comparar la eficacia del diagnóstico del ronquido por medio del RoncoLab contrastado con el PSQ. Materiales y Método: Estudio observacional, analítico, en el cual se incluyeron 31 niños de 7 a 11 años, que acudieron a la clínica de odontopediatría de la Benemérita Universidad Autónoma de Puebla, México (BUAP) Se aplicó el PSQ a los 31 niños para el diagnóstico de TRS, después se le instruyó al tutor como descargar y utilizar la aplicación móvil para registrar los datos obtenidos en las horas de sueño en casa. El análisis estadístico de concordancia entre los instrumentos diagnósticos se realizó con el Índice Kappa de Cohen a un nivel de confianza del 95. Resultados: El Índice de Kappa para la identificación de los ronquidos primarios fue de 0.743 (p<0.05). La sensibilidad de la aplicación fue de 0.92, mientras la especificidad fue de 0.82. Conclusión: Existe buena concordancia entre el PSQ y el RoncoLab en el diagnóstico de ronquido primario, con sensibilidad y especificidad aceptable.

A evolução da pandemia por Covid-19 em estados do Sul e Nordeste do Brasil: um estudo descritivo da resposta dos sistemas de saúde a partir da divulgação de dados epidemiológicos e das medidas de enfrentamento decretadas / The Covid-19 pandemic in the South and Northeast regions of Brazil: a descriptive study of the health system's response regarding the pandemic data dissemination and countermeasures decreed

Após a pandemia provocada pelo SARS COV-2 atingir a Europa, o Brasil tornou-se um dos epicentros. Considerando diferenças históricas nos indicadores de saúde entre as regiões Sul e Nordeste do país, regidas pelo mesmo sistema de saúde, este estudo tem como objetivo descrever a evolução inicial da pandemia nestas regiões a partir de informações das plataformas digitais das secretarias estaduais de saúde. Foram analisados número de casos, óbitos e testes realizados, de fevereiro de 2020 a maio de 2021. Os dados foram relacionados com as medidas de enfrentamento à pandemia tomadas pelos estados, coletadas a partir dos decretos de restrições e flexibilizações dos serviços e comércio. No Sul, o estado com mais casos foi Santa Catarina (13350/100.000hab), o Rio Grande do Sul teve maior taxa de mortalidade (246,8/100.000hab) e mais testagens (32378/100.000hab). No Nordeste, Sergipe teve mais casos (10216/100.000hab), Piauí mais testagens (23.917/100.000hab) e Ceará teve maior taxa de mortalidade (222,8/100.000hab). As medidas de enfrentamento ao coronavírus no Brasil não foram uniformes entre os estados, nem a aplicação das testagens, evidenciando a falta de coordenação nacional nas ações. [au]

After the pandemic caused by SARS COV2 reached Europe, Brazil became one of the epicenters. Considering historical differences in health indicators between the South and Northeast regions of the country, coordinated by the same health care system, this study aims to describe the early evolution of the pandemic in these regions based on information collected from state health secretariats online platforms. Data of numbers of cases, deaths and tests carried out, from 2020 to May 2021 were analyzed and related to measures to combat the pandemic by the states, based on decrees. In the South, the state with the most cases was Santa Catarina (13350/100,000 inhab), Rio Grande do Sul had the highest mortality rate (246.8/100,000 inhab) and more testing (32378/100,000 inhab). In the Northeast, Sergipe had more cases (10216/100,000 inhab) and Piau testing (23295/100,000 inhab), Ceará had higher mortality rates (222.8/100,000 inhab). The measures to combat the coronavirus in Brazil were not uniform between the states, nor testing, evidencing the lack of national coordination. [au]

Microencapsulation of Lactobacillus plantarum in W/O emulsions of okara oil and block-copolymers of poly(acrylic acid) and pluronic using microfluidic devices.

Okara oil is a by-product remaining from defatting okara, the solid residue generated after extracting the aqueous fraction of grounded soybeans in the elaboration of soy beverages. The goal of this work was to encapsulate the probiotic Lactobacillus plantarum CIDCA 83114 into W/O emulsions composed of a block-copolymer constituted of pluronic® and acrylic acid (PPP12) and okara oil, prepared in microfluidic devices. For comparative purposes, alginate was also included as a second dispersed phase. Lactobacillus plantarum CIDCA 83114 was suspended in PPP12 or alginate giving rise to dispersed phases with different compositions, named I, II, III and IV. Controls were prepared by suspending microorganisms in water as dispersed phase. 6-carboxyfluorescein was added as bacterial marker in all the emulsions. The presence of green dyed bacteria in the dispersed phases, inside the droplets of the emulsions and the absence of fluorescence outside them, confirmed the complete encapsulation of bacteria in the dispersed phases. After being prepared, emulsions were freeze-dried. The exposure to gastric conditions did not lead to significant differences among the emulsions containing polymers. However, in all cases bacterial counts were significantly lower than those of the control. After exposing emulsions to the simulated intestinal environment, bacterial counts in assays I, II and III (emulsions composed of only one dispersed phase or of two dispersed phases with bacteria resuspended in the PPP12 one) were significantly greater than those of the control (p < 0.05) and no detectable microorganisms were observed for assay IV (emulsions composed of two dispersed phases with bacteria resuspended in the alginate one). In particular, bacterial cultivability in emulsions corresponding to assay I (only PPP12 as dispersed phase) exposed to the intestinal environment was 8.22 ± 0.02 log CFU/mL (2 log CFU higher than the values obtained after gastric digestion). These results support the role of PPP12 as an adequate co-polymer to protect probiotics from the gastric environment, enabling their release in the gut, with great potential for food or nutraceutical applications.

Trastornos temporomandibulares en niños y su relación con la lactancia artificial con uso del biberón / Temporomandibular disorders in children and its relation to artificial lactation with bottle-feeding

Objetivo. Establecer la relación de los trastornos temporomandibulares (TTM) de niños de 8 a 10 años, con lactancia artificial exclusiva con uso del biberón. Métodos. Estudio de casos y controles, (pareados por edad y sexo). Se incluyeron 38 niños, sin alteraciones del crecimiento ni parafunciones bucales severas, previo consentimiento (madres) y asentimiento (niños) informado. Los participantes fueron diagnosticados con TTM (19 casos) o sin diagnóstico de TTM (19 controles) utilizando los criterios diagnósticos para los TTM (CD/TTM), previa estandarización de la investigadora; kappa inter 0,98 e intraobservador 0,73. Los antecedentes de lactancia fueron registrados con cuestionario ex profeso: tipo y tiempo de administración y datos generales del niño y la madre. Se calculó estadística descriptiva e inferencial; Ji cuadrada para identificar la asociación entre TTM y lactancia artificial exclusiva con uso del biberón, y razón de posibilidades (OR), con valor significativo ≤0,05. Resultados. Edad 8,95±0,84 años, 57,9% sexo masculino, sin diferencias por grupos en la edad y escolaridad de las madres (p>0,05). El tipo de TTM más común fue dolor muscular (57,9%), seguido de luxación del disco con reducción y su combinación (21,1%). La media de apertura bucal, sitios musculares y articulares doloridos fue estadísticamente diferente entre los casos y controles (p<0,05). El porcentaje de alimentación con lactancia artificial exclusiva con uso del biberón fue igual en ambos grupos (57,9% p=1,00), el OR calculado fue de 1 con IC95% de 0,27-3,60. Conclusión. No se encontró relación entre la lactancia artificial con el uso del biberón y los TTM. El tipo de TTM más frecuente fue dolor muscular. Palabras clave: Niños; Conducta en la lactancia; Asimetría facial; Trastornos de la articulación temporomandibular; Odontología pediátrica (fuente DeCS BIREME).

Objective. To establish the relationship of temporomandibular disorders (TMD) in children aged 8 to 10 years, with exclusive artificial feeding. Methods. Case-control study (matched by age and sex). 38 children were included, without growth disturbances or severe oral parafunctions, with prior informed consent (mothers) and assent (children). Participants were diagnosed with TMD (19 cases) or without TMD diagnosis (19 controls) using the Diagnostic Criteria for TMD (CD / TMD), after standardization by the researcher; kappa inter 0.98 and intraobserver 0.73. The history of breastfeeding was recorded with an express questionnaire: type and time of administration, and general data of the child and the mother. Descriptive and inferential statistics were calculated; Chi square analysis was performed to identify the association between TMD and exclusive artificial feeding, and odds ratio (OR), with a significant value of ≤0.05. Results. Age 8.95 ± 0.84 years, 57.9% male, with no differences in age or education of the mother between groups (p> 0,05). The most common type of TMD was muscle pain (57.9%), followed by disc luxation with reduction and combination (21.1%). The mean mouth opening, muscle and joint pain sites were statistically different between cases and controls (p <0.05). The percentage of exclusive artificial feeding was the same in both groups (57.9% p = 1.00), the calculated OR was 1 with a 95% CI of 0.27-3.60. Conclusions. Artificial feeding with the use of a bottle was not related to TMD. The most common type of TMD was muscle pain.

Release of health-related compounds during in vitro gastro-intestinal digestion of okara and okara fermented with Lactobacillus plantarum.

Okara is a highly perishable by-product remaining after filtration of the smashed soybeans seeds in the production of soymilk. Due to its nutritional value, different approaches have been developed to use it as functional ingredient. Fermentation of okara appears as an interesting strategy to preclude spoilage, providing a more stable matrix to be incorporated in the formulation of functional foods. Okara has antioxidant compounds but the effect of fermentation, and their bioaccessibility still need to be investigated. To achieve this aim, the phenolic compounds (as determined by TPC and TFC assays) and the antioxidant properties (as determined by ABTS ·+, DPPH · , O2 ·- assays) of okara and okara fermented with Lactobacillus plantarum CIDCA 83114 were assessed both before and after exposure to simulated gastro-intestinal conditions. Before digestion, okara showed higher values of TPC and TFC than the fermented counterpart. Although a decrease of TPC and TFC was observed after exposing okara to gastric conditions, no significant differences between okara and fermented okara were detected. No further decrease of TPC were observed in intestinal conditions. Okara showed higher antioxidant activity than fermented okara. There was a considerable decrease in the antioxidant activity for both samples when exposed to gastric and intestinal conditions. A good correlation between TFC and antioxidant activities was detected, suggesting that flavonoids play an important role as antioxidants. As a whole, this work provides a solid support for the stability of phytochemicals along the digestive process of both okara and fermented okara.

Relationship between carbohydrate composition and fungal deterioration of functional strawberry juices preserved using non-thermal treatments.

BACKGROUND: The quantification of the main carbohydrates present in strawberry juices enriched with inulin and fructo-oligosaccharides (FOS) and preserved by non-thermal techniques (vanillin and ultrasound) was conducted, in addition to an investigation of the evolution of these compounds and their relationship with fungal deterioration over 14 days of refrigerated storage. RESULTS: A simple and environmentally friendly analytical approach based on high-performance liquid chromatography with a reflection index detector was developed for simultaneous determination of inulin, FOS and mono- and disaccharides present in the juices. When analyzing the evolution of carbohydrates during storage, a direct relationship between the consumption of sucrose and the growth of yeasts and molds (main spoilage flora in strawberry) was observed, especially in untreated samples (control). By contrast, no sucrose consumption was observed during storage of the treated sample, thus demonstrating the efficiency of the non-thermal treatments for controlling yeasts and mold growth. In turn, inulin and FOS added to juices were not degraded during storage. CONCLUSION: The results obtained in the present study demonstrate that non-thermal treatments are adequate for preventing the growth of deteriorative flora in strawberry juices and that the addition of inulin and FOS can be a good strategy for functionalizing them, as well as improving their nutritional properties. © 2017 Society of Chemical Industry.

Nisin Z produced by Lactococcus lactis from bullfrog hatchery is active against Citrobacter freundii, a red-leg syndrome related pathogen.

Lactococcus lactis subsp. lactis CRL 1584 isolated from a bullfrog hatchery produces a bacteriocin that inhibits both indigenous Citrobacter freundii (a Red-Leg Syndrome related pathogen) and Lactobacillus plantarum, and Listeria monocytogenes as well. Considering that probiotics requires high cell densities and/or bacteriocin concentrations, the effect of the temperature on L. lactis growth and bacteriocin production was evaluated to find the optimal conditions. Thus, the growth rate was maximal at 36 °C, whereas the highest biomass and bacteriocin activity was achieved between 20 and 30 °C and 20-25 °C, respectively. The bacteriocin synthesis was closely growth associated reaching the maximal values at the end of the exponential phase. Since bacteriocins co-production has been evidenced in bacterial genera, a purification of the bacteriocin/s from L. lactis culture supernatants was carried out. The active fraction was purified by cationic-exchange chromatography and then, a RP-HPLC was carried out. The purified sample was a peptide with a 3353.05 Da, a molecular mass that matches nisin Z, which turned out to be the only bacteriocin produced by L. lactis CRL 1584. Nisin Z showed bactericidal effect on C. freundii and L. monocytogenes, which increased in the presence L-lactic acid + H2O2. This is the first report on nisin Z production by L. lactis from a bullfrog hatchery that resulted active on a Gram-negative pathogen. This peptide has potential probiotic for raniculture and as food biopreservative for bullfrog meat.

Frecuencia de reactividad a la prueba VDRL y de positividad para Chlamydia spp, VIH y virus de la hepatitis B y C en una institución prestadora de servicios de la salud de Medellín, 2015 / Frequency of VDRL reactivity and positivity for Chlamydia spp. HIV and Hepatitis B virus and C in an institution providing health services of Medellin, 2015

Resumen Introducción: En Medellín se desconoce la magnitud de diversas infecciones de transmisión sexual en estudios poblacionales. Objetivo: Determinar la frecuencia de reactividad a la VDRL y positividad para Chlamydia spp. y los virus de la inmunodeficiencia humana, hepatitis B y C en una institución prestadora de servicios de salud en Medellín 2015. Métodos: Estudio transversal en la totalidad de personas tamizadas para reactividad a la VDRL (N = 10 288), Chlamydia spp. (N = 349), VIH (N = 5 386) y virus de la hepatitis B (N = 3 369) y C (N = 1 450). Se utilizaron registros del laboratorio, se hizo control de sesgos por verificación de rangos y reproducibilidad en la extracción de datos. Se emplearon pruebas con sensibilidad y especificidad mayor al 95 %. Se estimó la frecuencia de cada agente y se exploró su asociación con sexo y grupo etario usando pruebas de Fisher y chi cuadrado. Resultados: El rango de edad fue 0-98 años, con una mayor proporción de mujeres. La frecuencia de Chlamydia spp. fue 3,4 %, VDRL reactivo 2,7 %, virus hepatitis B 1,7 %, virus hepatitis C 0,6 % y VIH 0,2 %. La ocurrencia de VDRL reactivo, VIH y virus hepatitis B fue estadísticamente mayor en hombres. El grupo etario presentó asociación con la frecuencia de VDRL reactivo, VIH y virus hepatitis B y C. Por su parte, la VDRL reactiva fue mayor en niños y las infecciones virales en los mayores de 40 años. Conclusión: La frecuencia global fue similar a la reportada en otras poblaciones, con excepción de Chlamydia spp. que fue menor en esta población; las frecuencias específicas evidencian divergencias en la distribución de los agentes, lo que permite la identificación de los grupos con mayor ocurrencia para orientar estudios posteriores y medidas preventivas.

Abstract Introduction: In Medellin, the magnitude of various sexually transmitted diseases is unknown based on population studies. Objective: To determine frequency of VDRL reactivity and positivity for Chlamydia spp. HIV, Hepatitis B virus (HBV) and Hepatitis C Virus (HCV) in an institution health of Medellin, 2015. Methods: a cross-sectional study was done in all people screened for VDRL (N = 10 288), Chlamydia spp. (N = 349), HIV (N = 5 386), Hepatitis B Virus (N = 3369) and Hepatitis C Virus (N = 1450). Clinical laboratory records were used, the bias control was realized through verification for ranges and reproducibility in data extraction. Tests with sensitivity and specificity greater than 95 % were used. The frequency of each agent was estimated and its association with sex and age groups were explored using Fisher and chi-square. Results: The age range was 0-98 years, with a higher proportion of women. The frequency of Chlamydia spp. was 3.4 % (95% CI = 1.4- 5.4), reactive VDRL 2.7% (95% CI = 2.4-3.0), Hepatitis B Virus 1.7% (95% CI = 1.2-2.1), Hepatitis C Virus 0.6% (IC95 = 0.1-1.0) and HIV 0.2% (95% CI = 0.1-0.3). The occurrence of reactive VDRL, HIV and HBV was statistically higher in men compared with the women. The age group presented association with reactive VDRL, HIV and Hepatitis B and C Virus. Reactive VDRL was higher in children and viral infections in patients over 40 years. Conclusion: The overall frequency was similar to that reported in other populations, except for Chlamydia spp., which it was lower in the current study; meanwhile, the specific frequencies show differences in the distribution of agents by sex and age group, allowing the identification of the groups with major occurrence, for orientation of further studies and preventive measures in specific groups.

Okara: A Nutritionally Valuable By-product Able to Stabilize Lactobacillus plantarum during Freeze-drying, Spray-drying, and Storage.

Okara is a nutritionally valuable by-product produced in large quantities as result of soymilk elaboration. This work proposes its use as both culture and dehydration medium during freeze-drying, spray-drying, and storage of Lactobacillus plantarum CIDCA 83114. Whole and defatted okara were employed as culture media for L. plantarum CIDCA 83114. The growth kinetics were followed by plate counting and compared with those of bacteria grown in MRS broth (control). No significant differences in plate counting were observed in the three media. The fatty acid composition of bacteria grown in whole and defatted okara showed a noticeable increase in the unsaturated/saturated (U/S) fatty acid ratio, with regard to bacteria grown in MRS. This change was mainly due to the increase in polyunsaturated fatty acids, namely C18:2. For dehydration assays, cultures in the stationary phase were neutralized and freeze-dried (with or without the addition of 250 mM sucrose) or spray-dried. Bacteria were plate counted immediately after freeze-drying or spray-drying and during storage at 4°C for 90 days. Freeze-drying in whole okara conducted to the highest bacterial recovery. Regarding storage, spray-dried bacteria previously grown in whole and defatted okara showed higher plate counts than those grown in MRS. On the contrary, freeze-dried bacteria previously grown in all the three culture media were those with the lowest plate counts. The addition of sucrose to the dehydration media improved their recovery. The higher recovery of microorganisms grown in okara after freeze-drying and spray-drying processes and during storage was ascribed to both the presence of fiber and proteins in the dehydration media, and the increase in U/S fatty acids ratio in bacterial membranes. The obtained results support for the first time the use of okara as an innovative matrix to deliver L. plantarum. Considering that okara is an agro-waste obtained in large quantities, these results represent an innovative strategy to add it value, providing a symbiotic ingredient with promising industrial applications in the development of novel functional foods and feeds.

Prevalencia de trastornos temporomandibulares en niños mexicanos con dentición mixta / Prevalence of temporomandibular disorders in Mexican children with mixed dentition

Objetivo Determinar y contrastar con la bibliografía reportada, la prevalencia de trastornos temporomandibulares con un instrumento validado en dentición mixta de niños mexicanos. Métodos Se incluyeron 150 niños de 8 a 12 años de edad, de cualquier sexo que asistieron a la clínica de estomatología pediátrica de la BUAP, evaluados con los criterios diagnósticos para la Investigación de los TTM (CDI/TTM) por investigador previamente estandarizado (kappa=0,93). Los resultados se contrastaron con lo reportado en la bibliografía. Resultados La prevalencia de TTM fue del 20,7%, predominantemente de tipo muscular (77,4%), el 33,3% presentó alteración del patrón de apertura bucal, el 34% presentó ruidos articulares (chasquido), la función mandibular más comprometida fue el masticar (6%). Estos resultados contrastan con lo reportado en la bibliografía, específicamente en los sitios musculares doloridos y las cefaleas, probablemente explicado por los diferentes instrumentos utilizados. Conclusión La prevalencia de TTM es contrastante entre diversos estudios, es necesario enfatizar la necesidad de evaluar éstos durante la niñez y la juventud con instrumentos validados.(AU)

Objective To determine and compare with reports in the bibliography, the prevalence of temporomandibular disorders with an instrument validated for Mexican children with mixed dentition. Methods 150 children, from 8 to 12 years of age and of any sex who attended the pediatric stomatology clinic of the BUAP (Meritorious Autonomous University of Puebla) were included and evaluated with the diagnostic criteria for research on TTM (CDI/TTM) by a researcher who had been previously standardized (kappa=0.93). The results contrasted with reports in the bibliography. Results The prevalence of TTM was 20.7%. It was predominantly muscular (77.4%), though 33.3% showed alteration of the mouth-opening pattern, 34% showed joint noises (clicks). The most compromised mandibular function was chewing (6%). These results contrast with reports in the bibliography, specifically in terms of muscle pain sites and headaches, probably explained by different instruments used. Conclusion The prevalence of TTM is in contrast among different studies. It is necessary to place emphasis on the need to evaluate these factors during the childhood and adolescence with validated instruments.(AU)

[Prevalence of temporomandibular disorders in Mexican children with mixed dentition]. / Prevalencia de trastornos temporomandibulares en niños mexicanos con dentición mixta.

Objective To determine and compare with reports in the bibliography, the prevalence of temporomandibular disorders with an instrument validated for Mexican children with mixed dentition. Methods 150 children, from 8 to 12 years of age and of any sex who attended the pediatric stomatology clinic of the BUAP (Meritorious Autonomous University of Puebla) were included and evaluated with the diagnostic criteria for research on TTM (CDI/TTM) by a researcher who had been previously standardized (kappa=0.93). The results contrasted with reports in the bibliography. Results The prevalence of TTM was 20.7%. It was predominantly muscular (77.4%), though 33.3% showed alteration of the mouth-opening pattern, 34% showed joint noises (clicks). The most compromised mandibular function was chewing (6%). These results contrast with reports in the bibliography, specifically in terms of muscle pain sites and headaches, probably explained by different instruments used. Conclusion The prevalence of TTM is in contrast among different studies. It is necessary to place emphasis on the need to evaluate these factors during the childhood and adolescence with validated instruments.

Espermocultivo: crecimiento bacteriano del eyaculado y su relación con los parámetros seminales / Spermoculture: bacterial growth of the ejaculate and its relationship with the seminal parameters

ANTECEDENTES: En el semen, algunos microorganismos pueden encontrar las condiciones óptimas para sobrevivir, ocasionando daños a los espermatozoides y desencadenando procesos de infertilidad o infecciones del tracto reproductivo. Entender el papel de los microorganismos aislados en el semen, contribuye a mejorar el diagnóstico de casos de infertilidad donde la única causa aparente son los procesos infecciosos. OBJETIVO: Describir y correlacionar los parámetros seminales y el crecimiento bacteriano del eyaculado. MÉTODOS: Identificación de los microorganismos aislados en 43 espermocultivos-clínicos y 28 espermocultivos-investigación. Se realizó conteo de las unidades formadoras de colonia a los espermocultivos-investigación y análisis de las características espermáticas. Resultados: Se obtuvo crecimiento bacteriano en 14 (32,6%) de los espermocultivos-clínicos y 15 (53,6%) de los espermocultivos-investigación. Los microorganismos aislados fueron Enterococcus faecalis, Escherichia coli, Morganella morganii, Staphylococcus spp coagulasa negativo, Klebsiella pneumoniae y microbiota mixta. En este estudio se observó abundante crecimiento de cocos aerobios. Finalmente, no se encontró asociación entre la disminución en la calidad de los parámetros seminales y los microorganismos. CONCLUSIONES: La presencia de bacterias en el semen no afecta la calidad seminal.

BACKKGROUND: Microorganisms can find the optimal conditions for survival in semen, causing damage to the spermatozoa and triggering processes of infertility or reproductive tract infections. Therefore, understanding the role of the microorganisms present in semen can help to improve the diagnosis of infertility cases where the only apparent cause is infectious processes. OBJECTIVE: To describe and correlate semen parameters and bacterial growth in ejaculate. METHODS: Identification of microorganisms isolated in 43 clinical spermocultures and 28 research spermocultures. We assessed colony-forming unit counts and sperm characteristics of research spermocultures. In addition, semen parameters were evaluated in each ejaculate. RESULTS: Bacterial growth was obtained in 14 (32.6%) of the 43 clinical spermocultures and 15 (53.6%) of the 28 research spermocultures. The isolated microorganisms were Enterococcus faecalis, Escherichia coli, Morganella morganii, Staphylococcus coagulase negative, Klebsiella pneumoniae and mixed microbiota. Finally, in this study a large growth of aerobic cocci was observed. We did not find association between the decline in the quality of semen parameters and microorganisms. CONCLUSION: The presence of bacteria in semen does not affect semen quality.

Asociación entre hábitos parafuncionales de la cavidad bucal y los transtornos temporomandibulares en adolescentes / Parafunctional habits of oral cavity and adolescents temporomandibular disordes

La musculatura del sistema masticatorio y la articulación temporomandibular (ATM) están protegidos por reflejos nerviosos básicos y sistema neuromuscular a través de la coordinación de fuerzas musculares, todo lo que produce sobrecarga muscular repetitiva como los hábitos parafuncionales (HPF) pueden ocasionar trastornos temporomandibulares (TTM) Los HPF se caracterizan por movimientos anormales a la función mandibular normal sin objetivo funcional, al estar alterados constituyen una fuente productora de fuerzas traumáticas caracterizadas por dirección anormal, intensidad excesiva y repetición frecuente y duradera (Rolando Castillo Hernández, 2001). El objetivo del estudio fue identificar la asociación entre la presencia de hábitos parafuncionales de la cavidad bucal y los TTM en adolescentes de la ciudad de Puebla. Estudio observacional descriptivo. Se incluyeron 258 adolescentes, 132 (51.2%) mujeres y 126 (48.8%) hombres, con una edad promedio de 12.5±.73 y quienes fueron diagnosticados con los CDI/TTM y los HPF fueron auto-reportados por los pacientes. Se encontró una prevalencia de los TTM del 39.9% y una prevalencia de HPF del 86%. Los HPF más frecuentemente reportados fueron la succión labial y la onicofagia. Se encontró una asociación significativa (x2=7.31, p=0.007) entre los hábitos parafuncionales y los TTM en adolescentes.

The muscles of the masticatory system and temporomandibular joint (TMJ) are protected by basic nerve reflex and neuromuscular system through the coordination of muscle forces, all that repetitive muscle overload occurs as habit parafunctional (HPF) can cause temporomandibular disorder TMD) The characteristics of HPF are abnormal jaw movements without a functional objective. Being the jaw movements altered, they constitute a source of traumatic forces with an abnormal direction, excessive intensity and long-lasting and frequent duration. (Rolando Hernandez Castillo 2001). Objective: was to identify the association between the presences of parafunctional habits of the oral cavity and TMD in adolescents in the Puebla city in Mexico. Material and methods: Is a observational study, we included 258 adolescents 132 (51%) females and 126 (48.8%) were men, mean age 12.5±.73 and who were diagnosed with CDI/TTM and HPF were self- reported by patients. Results: The prevalence of TMD was 39.9% and a prevalence of 86% HPF. The most frequently reported HPF were lip sucking and nail biting. We found a significant association (x2= 7.31, p = 0,007) between HPF and TMD in adolescents.

Características operativas de las pruebas de reacción en cadena de la polimerasa en tiempo real y la amplificación isotérmica mediada por Asas en la detección de tuberculosis pulmonar: una revisión sistemática / Operative characteristics of real time polymerase chain reaction and loop mediated isothermal amplification in the detection of pulmonary tuberculosis: a systematic review

Objetivo: exponer las características operativas de las pruebas de reacción en cadena de la polimerasa en tiempo real (qPCR) y la Amplificación isotérmica mediada por Asas (LAMP) para el diagnóstico de tuberculosis pulmonar causada por miembros del complejo Mycobacterium tuberculosis. Metodología: revisión sistemática de la literatura científica publicada durante los años 2005 a 2009, de la prueba LAMP comparada con la qPCR para el diagnóstico de tuberculosis pulmonar. Resultados: los hallazgos muestran sensibilidades de la prueba LAMP que fluctúan entre 94.1% – 100%, comparado con un 85.2% a 86.3% para la prueba qPCR, y unas especificidades entre 94.2 – 100% para LAMP frente a 88.6% – 100% de la qPCR. Conclusiones: estos hallazgos demuestran la importancia de evaluar dicha metodología en nuestra región para mejorar el diagnóstico de esta enfermedad.